RPG Seminar – Two photon microscopy based on structured optical beam illumination
04 Jun, 2021 (Fri)
4:00 pm
Webinar Link:

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Mr. Sabir Ul Alam
Department of Electrical and Electronic Engineering
The University of Hong Kong


Two-photon microscopy (TPM) has been an important tool in neuroscience, which necessitates functional imaging of neural cells buried deep inside the brain. To enhance the performance of TPM; over time structured optical beam illumination have gained great interest, due to its fast imaging capability, with high resolution and simple system design. Among the structured optical beams, the Bessel beam and Airy beam has been shown there imaging capability in two-photon microscopy. We have proposed two photon microscopies based on hollow Gaussian beam illumination, which can give higher resolution then the conventional TPM and additionally provide control over the PSF size both laterally and axially by varying the mode order of the beam, which is necessary for many applications. Moreover, TPM based on the Bessel beam provided single shot volumetric imaging that enhance the imaging speed. Conventionally generated, Gaussian beam illumination through axicon based Bessel beam is axially fixed in nature moreover, the central tip of the axicon create oscillation in the central lobe of the Bessel beam that create a disadvantage in imaging. We have proposed hollow Gaussian beam illuminated Bessel beam based TPM that can overcome the central lobe oscillation and additionally provide tuning in axial position.


Zoom Link (during COVID-19 special period):

Meeting ID: 968 1812 4124
Password: 381740


Biography of the speaker:

Sabir Ul Alam received the B.Tech degree in Electronics and Communication Engineering from Aliah University, Kolkata, India in 2015 and M.Tech degree in Optics and Optoelectronics from the department of Applied Optics and Photonics from University of Calcutta, Kolkata, India in 2017. He worked as a research assistant in Physical research laboratory (ISRO), Ahmedabad, India. He is currently a PhD student at the department of Electrical and Electronic Engineering of University of Hong Kong. His research interests include optical beam shaping, Two photon microscopy system.

All are welcome!


Prof. K.K.Y. Wong

Most seminars are open to the general public, free of charge, unless otherwise stated. Registration is not required. Arrangement for car parking facilities on campus please contact us for details.

For enquiries, please contact:
Department of Electrical and Electronic Engineering,
Room 601, Chow Yei Ching Building,
Pokfulam Road, Hong Kong
Tel: (852) 3917 7093
Email: seminar@eee.hku.hk